Generator
Bgal alpha

Part:BBa_K1477014:Design

Designed by: 2014 Ivy Tech South Bend IN team   Group: iGEM14_IvyTech_SouthBendIN   (2014-08-21)


Constitutive promoter +RBS+LacZ alpha+Term.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Registry BBa_J23102 and BBa_I73202


References

Langley, K.E., M.R. Villarego, A.V. Fowler, P.J. Zamenof and I. Zabin. 1975. Molecular Basis of beta-galactosidase alpha-complementation. Proc. Natl. Acad. Sci. 72(4): 1254-1257.